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- Biñan City: A commercial center famous for Puto Biñan.
- Cabuyao City: Known as the “Enterprise City of the Philippines.”
- Calamba City: The birthplace of national hero Dr. Jose Rizal.
- San Pablo City: Famous for its seven scenic crater lakes.
- San Pedro City: The northern gateway to the Laguna province.
- Santa Rosa City: A major industrial, automotive, and technology hub.
- Alaminos: Noted for its agricultural lands and Hidden Valley Springs.
- Bay: One of the oldest settlements and former provincial capital.
- Calauan: Famous for its sweet pineapples and Pineapple Festival.
- Los Baños: A nature and science town hosting Mt. Makiling.
- Pila: Well-preserved town featuring Spanish-era heritage houses.
- Victoria: Recognized as the “Duck Raising Capital of the Philippines.”
- Lumban: The “Hand-Embroidery Capital of the Philippines.”
- Mabitac: Site of the historic Battle of Mabitac.
- Pagsanjan: Renowned for the famous Pagsanjan Falls rapids boat ride.
- Pangil: Eco-tourism destination featuring natural river parks.
- Paete: Celebrated as the “Carving Capital of the Philippines.”
- Pakil: Home of the Turumba Festival and music heritage.
- Santa Cruz: The administrative capital and bustling commercial center.
- Siniloan: An educational and industrial hub for the northeast.
- Famy: The smallest municipality in the province by area.
- Kalayaan: Home to the Kalayaan Pumped-Storage Power Plant.
- Pangil: Known for its clean rivers and eco-parks.
- Santa Maria: High-altitude town producing citrus fruits and vegetables.
- Siniloan: Serves as the center of education in northeastern Laguna.
- Cavinti: Famous for underground caves and man-made eco-lakes.
- Luisiana: Called the “Little Baguio of Laguna” for its cold weather.
- Liliw: Widely popular for its affordable, quality footwear industry.
- Nagcarlan: Home to the unique Underground Cemetery historical landmark.
- Majayjay: Located at Mt. Banahaw’s foot, famous for cold springs.
- Rizal: A quiet, landlocked agricultural town harvesting coconuts.